Product Overview
Bacterial canker is the most important bacterial disease of tomato. The causal organism is Clavibacter michiganensis subsp. michiganensis (Cmm) and this bacterium can be intro-duced by contaminated seeds. For the detection of Cmm; toma-to seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. After the concentra-tion of the bacteria; dilution plating on two semi-selective media is performed. SCM medium is such a semi-selective media. Actually; there are several modifications in use con-cerning the used carbon source; LiCl and the addition of antibi-otics. This medium is used in combination with D2ANX medi-um (D5128). After dilution plating suspected isolates are trans-ferred to YDC. Finally the identity of suspected isolates is determined by a pathogenicity test or PCR. The colonies of Clavibacter michiganensis subsp. michiganensis on SCM are small; light to dark grey; glistening; fluidal and often irregularly shaped. For detailed information about DuchefaÂ’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure sectionÂâ€? at the home page of this website.