Product Overview
The MT (Milk-Tween) Medium is a semi-selective medium for the detection of Pseudomonas syringae pv. syringae (Pss); Pseudomonas savastanoi pv. phaseolicola (Pspha) and Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed. The medium relies on the ability of the micro-organisms to hydrolyze casein. Suspected isolates are transferred to YDC (Xap) or KB (Pss and Pspha). Finally; the identity of suspected col-onies is determined by PCR or a pathogenicity test.The colonies of Pspha and Pss are cream white; flat circular; 4-5 mm in diameter and produce a blue fluorescent pigment under UV light. Xap colonies (3 – 3.5 mm in diameter) are yellow; non fluorescent and typical two zones surround colonies: a bigger; clear zone of casein hydrolysis and a smaller zone of Tween 80 lipolysis. Xap var. fuscans (1 – 2 mm in diameter) produces a brown pigment within 5 days. For detailed information about DuchefaÂ’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure sectionÂâ€? at the home page of this website.