CAS number 369-07-3
Molecular weight C12H15NO8 = 301.3
Assay > 90 %
ONPG is a colorimetric and spectrophotometric substrate for detection of ß-galactosidase activity. ONPG is cleaved by ß-galactosidase via hydrolysis at the ß-1-4-glycosidic bond be-tween 2-nitrophenol and galactose. The released 2-nitro-p-henol is measured spectrophotometrically at 405 nm. The absorbance intensity at this wave-length is directly related to the specific activity.